Reactions of kia helps to includeexclude particular bacterial isolate in the family enterobacteriaceae. Page 25 material safety data sheet lewis university. Kligler iron agar kia or the identificaiton of enteric. Distribute into test tubes and sterilize at 121c for 15 minutes. Bailey and lacey substituted phenol red for andrade indicator previously used as ph indicator 3. Reactions in kligler iron agar medical laboratories.
Since there is only a small amount of sugar dextrose in the medium, acid production due to its fermentation is very limited and. Kligler iron agar and triple sugar iron agar suspicious colonies should be carefully picked from plating media to a screening medium such as kligler iron agar kia or triple sugar iron agar tsi or to any nonselective agar medium and then incubated overnight. Kligler iron agar is used for the differentiation of microorganisms in the enterobacteriaceae family on the. If the oxidase test could be reliably performed on growth from the surface of kligler s iron agar andor triple sugar iron agar slants, it would aid in the screening of potential stool pathogens.
Home bacteriology reactions in kligler iron agar reactions in kligler iron agar. Quality control appearance light yellow to pink homogeneous free flowing powder gelling firm,comparable with 1. The medium is recommended for the identification of colonies picked off from plating media such as macconkey agar, bismuth sulphite agar, or desoxycholate citrate agar, etc. Dispense into tubes and sterilize in autoclave at 121. Kligler iron agar is a combination of the lead acetate medium described by kligler 9 and. The production of acid by lactose andor dextrose fermentation results in color. If the pdf does not display below, you may also download it. Many members of the familyvibrionaceae have been implicated as causative agents of diarrhea. Stewart 1 nature volume 183, pages 1537 1538 1959 cite this article. The medium is recommended for the identification of colonies picked off from plating media such as macconkey agar, bismuth sulphite agar, or desoxycholate. Kligler iron agar kia combines features of kliglers lead acetate medium and russells double sugar agar.
Israel jacob kligler 24 april 1888 23 september 1944 was a microbiologist. Kliglers iron agar kia and bile esculin flashcards quizlet. Kligler iron agar intended use kligler iron agar is used for the identification of enterobacteria by the rapid detection of lactose and glucose fermentation with or without gas production, as well as the production of hydrogen sulfide. He was one of the first four professors of the hebrew university and the founder of department of hygiene and. Kliglers iron agar kia and bile esculin flashcards. Winslow, maine 04901 technical product information kligler iron agar catalog no t1478 16 x 125 mm tube t1479 16 x 125 mm slip cap intended use. Kligler iron agar is an agar medium used as a differential medium for the presumptive identification of the enterobacteriaceae based upon the fermentation of dextrose, lactose, and hydrogen sulfide production. Kligler iron agar is a combination of the lead acetate medium described by kligler 8 and. To inoculate triple sugar iron tsi agar or kligler s iron agar kia, the laboratory scientist should pick a wellisolated colony with an inoculating needle and stab the butt almost all the way to the bottom of the tube, then move the needle back and forth over the surface of the slant all the way to the top of the tube. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle. The original medium was a soft nutrient agar containing dextrose, andrade indicator and lead acetate. Bailey and lacey further modified the medium by substituting phenol red indicator for. A differential medium for the recognition of enteric pathogens by their ability to ferment glucose andor lactose, and liberate sulphides. A medium for the identification of enterobacteriaceae, based on double sugar fermentation and hydrogen sulphide production.
Heat to boiling, distribute and autoclave at 121c for 15 minutes. Kligler iron agar is a modification of kligler s original formula, recommended to identify pure cultures of colonies picked from primary plating media. Summary and explanation in 1918, kligler described a medium for detection of h2s and differentiation of. Kligler iron agar, in addition to peptone, hm peptone b and yeast extract, contains lactose and glucose dextrose, which enables the. Make a suspension of 52 g in 1 l of distilled water. The kliglers iron agar test employs a medium for the identification of enterobacteriaceae, based on double sugar fermentation and hydrogen sulphide production.
Remel kligler iron agar kia is a solid medium recommended for use in qualitative procedures for differentiation of enteric gramnegative bacilli on the basis of dextrose and lactose fermentation and hydrogen sulfide h2s production. Learn vocabulary, terms, and more with flashcards, games, and other study tools. The phenylalanine test on kliglers iron agar for the identification of. Bacterial agents of enteric diseases of public health concern. Kligler iron agar is a combination of the lead acetate medium described by kligler 1 and russels double sugar agar 2 and is used as a differentiation medium for typhoid, dysentery and allied bacilli 3. In 1918, kligler described a medium for detection of h 2 s and differentiation of salmonella spp. Other notes components gl peptic digest of animal tissue 15. Mix well and dissolve by heating with frequent agitation. Agar is used for the differentiation of members of. Oxidase testing from kliglers iron agar and triple sugar. Oct 06, 2018 kligler iron agar principles,composition and slants interpretation 3 likes comment share kligler iron agar abbreviated kia is a growth media recommended for the differential identification of gramnegative enteric bacilli from clinical and non clinical samples based on the fermentation sugars that is dextrose, lactose and the production of. Apr 03, 2018 for the love of physics walter lewin may 16, 2011 duration.
This can happen because utilization of sucrose in tsi agar suppresses the enzymic pathway that result in h2s production. Kliglers iron agar test procedure, uses and interpretation. History in 1911, russell described a medium containing two sugars for the isolation of typhoid bacilli in urine. Pick a single colony from the surface of a selective plating medium and smear a macconkey agar cm0007 plate. The tsi slant is a test tube that contains agar, a phsensitive dye phenol red, 1% lactose, 1% sucrose, 0. Edwards and ewings identification of enterobacteriaceae, 4th ed. Kligler iron agar kligler hajna is a solid culture medium for the identification of enterobacteriaceae, based on the fermentation of dextrose and lactose and the production of hydrogen sulfide. Kligler iron agar dehydrated culture media for microbiology preparation suspend 52 grams of the medium in one litre of distilled water.
Escherichia coli is an aerobe and facultative anaerobe. Heat stirring constantly to boiling point and boil for 1 minute. Kligler s iron agar or triple sugar iron agar kia and tsi are carbohydratecontaining screening media widely used in diagnostic microbiology. Kligler s iron agar kia is used for the detection of carbohydrate fermentation. Kligler iron agar principles,composition and slants. Kligler iron agar 7140 7140a kligler iron agar is used for the differentiation of microorganisms on the basis of dextrose and lactose fermentation and hydrogen sulfide production in a laboratory setting. Kligler iron agar 7140 intended use kligler iron agar is used for the differentiation of microorganisms on the basis of dextrose and lactose fermentation and hydrogen sulfide production. Select at least one of each colony type of the wellisolated colonies on each plate. While similar in use, the two media vary in the carbohydrates they contain. Description kligler iron agar is a solid medium used to distinguish between enterobacteriaceae. The salmonella screen consisted of kligler iron agar kia, motilityindoleureatryptophandeamination semisolid medium miutda, and the onitrophenylbetadgalactopyranoside onpg test. The tsi slant is a test tube that contains agar, a phsensitive dye, 1% lactose, 1% sucrose, 0.
The phenylalanine test on kligler s iron agar for the identification of proteus d. Product summary and explanation kligler iron agar is a modification of kliglers original formula, recommended to identify pure cultures of colonies picked. Specimen include urine, pus, faeces, cerebrospinal fluid infants, and blood for culture or depending upon site of infections. If the pdf does not display below, you may also download it here. Kligler iron agar dehydrated culture media for microbiology physical data. Most of these organisms are nonlactose fermenters, and all are oxidasepositive. For the love of physics walter lewin may 16, 2011 duration. Recommended as a differential medium in the study of the gramnegative intestinal microorganisms. The phenylalanine test on kliglers iron agar for the identification of proteus. Sugar fermentation is shown by acid production, which turns the indicator from red to yellow.
Download msds pdf search msds currently available for download bioworld offers kligler iron agar for your research at low price. Box 788 waterville, maine 049030788 227 china road winslow, maine 04901 technical product information kligler iron agar catalog no t1478 16 x 125 mm tube t1479 16 x 125 mm slip cap intended use. Print msds kligler iron agar, dehydrated download pdf. Kligler iron agar is a combination of the lead acetate medium described by kligler 9 and russels double sugar agar 7 and is used as a differentiation medium for typhoid, dysentery and allied bacilli 3.
A zionist and humanist, he was born in the austrohungarian empire, educated in the united states and spent most of his career in mandatory palestine, but died before the creation of the state of israel. Material safety data sheet section i product information product name or identity. Kligler iron agar kia combines features of kligler s lead acetate medium and russells double sugar agar. Kligler agar msds material safety data sheet or sds, coa and coq, dossiers, brochures and other available documents. Kliglers iron agar or triple sugar iron agar kia and tsi are carbohydratecontaining screening media widely used in diagnostic microbiology. Start studying kliglers iron agar kia and bile esculin.
The phenylalanine test on kliglers iron agar for the. Klingler iron agar combines the features of kligler s lead acetate medium 1,2,3 and russells double sugar agar 4. Those bacteria that ferment any of the three sugars in the medium will obviously produce byproducts. Kligler agar for the identification of gramnegative intestinal bacteria find msds or sds, a coa, data sheets and more information. The current formulation of kligler iron agar combines features of kligler s lead acetate medium with those of russells double sugar agar. Oxoid kligler iron agar based on the original medium 1,2,3 combines the principles of russell 4, double sugar agar, with ferric citrate as an indicator to detect hydrogen sulphide production.
Principle, procedure and results kliglers iron agar kia is used for the detection of. The growth characteristics of enterobacteriaceae on tsi media are slightly different from those on kligler iron agar. This can happen because of sucrose in the medium, which suppresses the enzymatic pathway that results in h. Rapid biochemical screening for salmonella, shigella. Hajna developed the formulation for tsi agar by adding sucrose to the double sugar dextrose and lactose formulation of kligler iron agar. Kligler iron agar, in addition to casein and meat peptones, contains lactose and dextrose which enable the differentiation of species of enteric bacilli due to color changes of the phenol red ph indicator in response to the acid produced during the fermentation of these sugars. Dehydrated culture media of pinkish beige colour preparation. Cool in a slanting position to obtain deep butts and short slopes. Kligler iron agar abbreviated kia is a growth media recommended for the differential identification of gramnegative enteric bacilli from clinical and non clinical samples based on the fermentation sugars that is dextrose, lactose and the production of hydrogen sulphide. Kligler iron agar is used for the differentiation of microorganisms in the enterobacteriaceae family on the basis of dextrose and lactose fermentation and hydrogen sulfide production. Kligler iron agar dehydrated culture media for microbiology. Kligler iron agar kia, in reaction tubes, inoculated with escherichia coli and. S production on kligler agar prod no 60787 but not on triple sugar iron agar. Start studying kligler s iron agar kia and bile esculin.